Greenland Agri Biotech (GAB) is not just production & supplier of tissue culture plants. We put great emphasis to provide different services as well. Due to over 12 years of experience and our network of specialized partners those who have 20 years of experience and experts in this field, we have created an extended knowledge base that we use to find the best possible solutions for you to setup your tissue culture laboratory and how to run the laboratory in proper planning to meet your target production and to give ultimate marketing solutions of your production and also sales. We provide consultancy services for setting up a plant tissue culture laboratory. Our team of expert professionals has long term experience in this domain and uses latest technology and resources for executing all the projects efficiently. Further, our team maintains a regular touch with the customers to understand their requirements.
The market for tissue culture plant production companies is increasingly competitive and companies should focus on their customers and brand with a marketing plan. Let your plants breathe life into your customers and let the GAB team grow new business for your tissue culture plants. it's crucial to understand the latest consumer and market trends. With over 12 years of expertise in market research in this industry, we have built one of the largest consumer networks in India. This extensive network enables you to efficiently and effectively find your desired target audience and reach to them.
GAB offers a complete suite of Market Entry services inclusive of all elements of a market feasibility study to help your business venture into a particular market. Our team of professionals offers support in every aspect of market entry strategy including different state government tenders according to your specific business needs. We will take time to truly understand your unique challenges and tailor our solutions accordingly.
Plant tissue culture has been a booming field of science over the last few decades. Now a day, its knowledge is so readily available that anyone with genuine interest can do it. In the long run, it can generate enormous financial returns. However, the first step is to set up a tissue culture laboratory. A lot of you might be curious about how you can set up your own tissue culture laboratory. What are the requirements? What equipment do you need to have? Where you need to be careful while planning a setup?
Tissue culture plants are quite useful because they are uniform and you can obtain high quality plants in relatively short duration. Also, if you are thinking of setting up a tissue culture facility for research or commercial purposes on a small to large-scale production levels, you can always contact our wonderful team for consultation and guidance. We specialize in designing and setting up plant tissue culture laboratories.
For now, let us talk about a basic commercial laboratory set up.
In order to set up your plant tissue culture laboratory, it is important that you carefully choose the location. You should try not to build it close to a facility which deals with micro-organisms (bacteria, fungi and so on). You should also try that it should not be in the close vicinity of other laboratories which handle insects. Another important point is that the lab should be away from storage places which store seeds and old plant material. However, if you cannot avoid these then you need to take extra precautions to keep the lab free from contamination.
For any tissue culture lab, there are certain basic requirements or facilities that you need to install for efficient functioning and experimentation. These are: -
Let us dive a bit deeper into these areas of a plant tissue culture lab.
This area is essential to prevent contamination coming from outside along with humans and others sources. It can be present only in the beginning of the lab or both in the beginning and exit of a lab. This area is primarily for you to change dresses and shoes. There should be separate shoes and dresses for you to use in the lab..
You need this area to wash the explants or other plant materials. You also need this to clean all your bottles, beakers, cylinders and flasks which you will use for media preparation and measuring ingredients. This area consists of large sinks and draining boards, bottle washing machines etc..
You need this area for preparation and for storage of chemicals. This area also has pH meters, TDS meter, weighing balances, R.O System and freeze. You can also choose to have a separate area for the media dispensing unit which is connected to autoclave area. The important thing to know is the equipment we need for media preparation which are:.
Media preparation is a crucial step, and you need to develop a system to have a step-by-step routine careful measurement of chemicals and preparation of media..
This area is used to transfer autoclaved media bottles through pass box and store just after autoclave to make the media cool and solid.
The area where media bottles need to incubate for 3 to 5 days after autoclave to observe the contamination status of media before inoculation of culture
The area where the process of IPQC/ quality control follows before the issue of culture stocks in inoculation room for further process. The expert will screen the fungal and bacterial growth in media and culture bottles both before issue for further process.
In ideal conditions, a separate room should be made. However, you need this area to have complete sterile conditions in order to transfer explants to media. So the activities for this room are:.
One of the most important key points for this area is that it should always have positive pressure airflow. You need positive pressure airflow to make sure the sterility label in the room inside.
For making transfers, you need to install laminar flow cabinets as well. This is to ensure constant flow of bacteria-free filtered air where you can transfer the culture in the cabinet.
After transferring explants in media, you need to store in ideal conditions for efficient growth and development. This means that you need to provide controlled temperature, humidity, air circulation, light quality, and duration of light. You should know that an automated climate and air filtration system works best for regulating these conditions.
These environmental factors are important for the plant cells to grow and differentiate into healthy shoots and roots at later stages. These specific conditions differ with each plant species. Hence, you need to be careful with providing the necessary growth environment for optimum development of tissue culture plantlets.
The area where rooted plants are washed and grade based on standard height and quality before planting in green house or despatch for sales.
Decontamination process is a vital part of tissue culture process. All contaminated and rejected media, culture bottles and other contaminated materials during quality control process will be autoclave to kill the bacterial and fungal growth. All decontaminated bottles and other materials have to be washed following the SOP to make the bottles and other materials ready for further process. This area should be very close to bottle washing area.
Developing tissue culture protocols for different plant species and varieties involves a lot of trial-and-error experiments. You will be experimenting with culture media recipes for different growth stages and also with different growth conditions in the growth room. Thus, you need this area to have efficient record keeping practices for different kind of tissue culture experiments. Hence, getting yourself a desktop computer for writing up reports and analysing the data will be a great idea.
So, with these areas in place, a tissue culture laboratory can be efficiently set up with productive workflow. If you are setting up a lab for commercial production, then you also need to focus not just on product workflow, but also on people workflow.. Apart from this, research and development of tissue culture protocols for each plant species also play a key role in the success of your plant tissue culture work and production.
We Greenland Agri Biotech provide consultancy services for setting up a plant tissue culture laboratory. Our team of expert professionals has long term experience in this domain and uses latest technology and resources for executing all the projects efficiently. Further, our team maintains a regular touch with the customers to understand their requirements.
We assist in the establishment of plant tissue culture laboratory as per NCSTCP (DBT) standards. We will help to identify the need for a plant tissue culture laboratory. We can customize your laboratory as per your requirement. After the establishment of your laboratory, we will train and coordinate you for the production of your plants. We will provide services for project proposals, establishment of commercially viable Unit, and financial analysis of the tissue culture laboratory, selection and purchase of chemicals and mother cultures.
Using our approach, you can increase the culture multiplication rate and uniformity with quality plants.
Whether you are experienced with tissue culture or new to the plant tissue culture world, we provide everything to build a complete cutting-edge tissue culture laboratory. We provide a turnkey solution from the design of your facility to climate-controlled multilayer propagation rooms, labour-saving automation, worker equipment, growing consumables and in-house training.
We design a complete layout for your plant factory. We have extensive experience creating the most efficient tissue culture laboratories for you.